The hyperglycemic neuropeptide of the terrestrial isopod, Porcellio dilatatus . II. Immunocytochemical demonstration in neurosecretory structures of the nervous system
Identifieur interne : 001763 ( Main/Exploration ); précédent : 001762; suivant : 001764The hyperglycemic neuropeptide of the terrestrial isopod, Porcellio dilatatus . II. Immunocytochemical demonstration in neurosecretory structures of the nervous system
Auteurs : G. Martin [France] ; P. P. Jaros ; G. Besse [France] ; R. KellerSource :
- General and Comparative Endocrinology [ 0016-6480 ] ; 1984.
Abstract
By use of an rabbit antiserum raised against the crustacean hyperglycemic hormone (CHH) of the shore crab, Carcinus maenas, CHH-producing perikarya were detected in the brain of the isopod, Porcellio dilatatus, by the double-antibody immunofluorescence technique. The course of the secretory axons to the neurohemal organ, the sinus gland, could be traced. The sinus gland also exhibited strong fluorescence. The reaction was very specific, no other structures gave appreciable immunofluorescence. There are two CHH cells in each hemisphere of the brain, located in the medio-rostral part of the protocerebrum. This area appears to correspond to the medulla terminalis of decapods. The cells are aldehyde fuchsin-positive and have been previously described as β1 cells. The immunofluorescence could be inhibited by preabsorption of the antiserum with pure Carcinus-CHH.
Url:
DOI: 10.1016/0016-6480(84)90104-7
Affiliations:
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The course of the secretory axons to the neurohemal organ, the sinus gland, could be traced. The sinus gland also exhibited strong fluorescence. The reaction was very specific, no other structures gave appreciable immunofluorescence. There are two CHH cells in each hemisphere of the brain, located in the medio-rostral part of the protocerebrum. This area appears to correspond to the medulla terminalis of decapods. The cells are aldehyde fuchsin-positive and have been previously described as β1 cells. The immunofluorescence could be inhibited by preabsorption of the antiserum with pure Carcinus-CHH.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Poitou-Charentes</li></region><settlement><li>Poitiers</li></settlement><orgName><li>Université de Poitiers</li></orgName></list><tree><noCountry><name sortKey="Jaros, P P" sort="Jaros, P P" uniqKey="Jaros P" first="P. P." last="Jaros">P. P. 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